You can get step-by-step instructions on how we evolve fluorescent proteins in bacteria & purify large amounts of smURFP!
https://doi.org/10.1007/978-1-0716-2667-2_4
The Erik A. Rodriguez Group at The George Washington University
Developing Tools to Image Molecules in Single Cells to Disease in Humans
You can get step-by-step instructions on how we evolve fluorescent proteins in bacteria & purify large amounts of smURFP!
https://doi.org/10.1007/978-1-0716-2667-2_4